Bio-inspired Microscale Topographies on Drie Defined Titanium Surface for Soft Tissue Regeneration in Implant Dentistry

TITANIUM SURFACE FOR SOFT TISSUE REGENERATION IN IMPLANT DENTISTRY Feifei Mao, Nannan Li, Shuhui Chen, Yifei Zhang, Shu He, Shicheng Wei and Jing Chen Academy for Advanced Interdisciplinary Studies, Peking University, China Institution of Micro/Nano electronics, Peking University, China : Both authors contributed equally to this work ABSTRACT Different patterns on Ti substrate which mimic the extracellular matrix (ECM) and cell morphology were designed to study the interaction of fibroblast (L929) and bacteria (Porphyromonas gingivalis) on the surface in vitro system. Patterned titanium samples were prepared by chlorine DRIE (Deep Reactive Ion Etch) to obtain expected microstructures. Patterned titanium samples were seeded with L929 and P. gingivalis for a period of 1-4days. It was revealed that the cell adhesion rates were more than doubled with promoted cell proliferation on the ECM-mimicked titanium surfaces. In addition, the bacterial adherence rates were greatly reduced on ECM surface. The bio-inspired titanium interface provides an excellent solution for soft tissue regeneration around the dental abutment.